Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550
- 貨號 Cat. No.
- AAR-011-AO
- 規格 Size
- 50 μl
Description
Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550
| Product#: | AAR-011-AO |
| Sizes: |
| 50 µl |
| Host: |
| Rabbit. |
| Epitope: |
Peptide NSSTEDGIKRIQDDC corresponding to amino acid residues 4-18 of human AT1 (Accession P30556). |
 |
| Putative epitope location: |
Extracellular, N-terminal domain.
|
| Homology: | ||
Mouse, rat, rabbit, dog- identical.
|
| Reactivity Confirmed: |
| Human, rat, mouse. |
Applications:
| Immunohistochemistry: |
Immunohistochemical staining of AT1 receptor in rat kidney. |
 |
Paraffin embedded section of rat kidney showing the most inner layer of the cortex. Notice that intense stain is present in proximal tubes (P) but not in collecting ducts (C) in the cortical labyrinths (CL). Also note that no staining is present both in thin portions of the Loop of Henle or in the collecting ducts in the medullar rays (MR). Slides were treated with citrate for antigen retrieval and then incubated overnight at 4èš“ with Anti-AT1 Receptor (extracellular)-ATTO-550 (#AAR-011-AO) (1:50)(Red). Nuclei are visualized with Hoechst 33342 (blue).
|
| Immunocytochemistry: |
| Immunocytochemical staining of live intact rat C6 glioma cells with Anti-AT1 receptor (extracellular)-ATTO- 550 (#AAR-011-AO). |
 |
Staining of live intact rat C6 glioma cells with Anti-AT1 receptor (extracellular)-ATTO-550 (#AAR-011-AO) antibody 1:50 (left). Right panel shows live view of the same field. |
Angiotensin II receptor type 1 or AT1 is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II).
Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration.
In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc.
As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.
Alomone Labs has developed a new antibody specific for AT1. The antibody was raised against a highly conserved epitope located at the N-terminus extracellular region of the human AT1 receptor. The Anti-AT1 receptor antibody (#AAR-011) has been tested in several applications including indirect flow cytometry, immunocytochemistry, immunohistochemistry and Western blot analysis.
We are pleased to offer a new version of this antibody that is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine.
The antibody has been tested in immunocytochemical and immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.