Anti-AMPA Receptor 3 (GluR3) (extracellular)
- 貨號 Cat. No.
- AGC-010
- 規格 Size
- 50 μl 0.2ml
Description
Anti-AMPA Receptor 3 (GluR3) (extracellular)
| Product#: | AGC-010 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
Rabbit. |
| Epitope: |
Peptide (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat AMPA Receptor 3 (Accession P19492 ). |
 |
| Putative epitope location: |
Extracellular, N-terminus. |
| Homology: |
Mouse, human, dog -identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat cerebellum lysate: 1. Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010)antibody (1:400). 2. Anti-AMPA Receptor 3 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Expression of AMPA Receptor 3 in mouse cerebellum. Immunohistochemical staining was performed in perfusion-fixed free floating sections (frozen). A. Distribution of AMPA Receptor 3 (red), was visualized by staining with Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010) in mouse cerebellum. B. Distrubiton of glial fibrillary acidic protein (green). C. Merge of the two images indicates that AMPA Receptor 3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). Nuclear counterstain is shown in blue. |
| Immunoprecipitation: |
 | Immunoprecipitation of rat cerebellum lysate with Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010) antibody:
Black arrow indicates the AMPA Receptor 3 (GluR3) protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010). |
| References: |
| 1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7. |
| 2. Lynch, M.A. (2004) Physiol. Rev. 84, 87. |
| 3. Steenland, H.W. et al. (2008) Eur. J. Neurosci. 27, 1166. |
L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors).
The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors.
The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively.
The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found.
AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1
Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential.
Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2
The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3
Alomone labs is pleased to offer a highly specific Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010) antibody directed against a well conserved epitope located in the extracellular N-terminal region of the rat AMPA Receptor 3. The antibody does not cross react with the related AMPA Receptor 2 subunit.
Anti-AMPA Receptor 3 (GluR3) (extracellular) (#AGC-010) antibody can be used in Western blot, immunohistochemical, and immunoprecipitation applications and will recognize AMPA Receptor 3 from rat, mouse, human and dog samples.