Anti-AMPA Receptor 2 (GluR2) (extracellular)-ATTO-488

貨號 Cat. No.

AGC-005-AG

規格 Size

50 μl

Description

 

Anti-AMPA Receptor 2 (GluR2) (extracellular)-ATTO-488

Product#:

AGC-005-AG

Sizes:

50 µl

Host:

Rabbit.

Epitope:

Peptide NVGNINNDKKDETYR(C), corresponding to amino acids 179-193 of rat AMPA Receptor 2 (Accession P19491).

Putative epitope location:

Extracellular, N-terminus.

Homology:

Mouse -identical; Human -14/15 residues identical.  Label: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range.. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC

Applications:

Immunohistochemistry:

Immunohistochemical staining of AMPA Receptor 2 (GluR2) in mouse brain

Distribution of GluR2 in the molecular layer of the mouse cerebellum. Frozen free-floating sections were stained with Anti-AMPAReceptor 2 (GluR2)(extracellular)-ATTO-488(#AGC-005-AG) antibody (1:20). Both dendrites of Purkinje cells (horizontal arrows) were stained (green). DAPI counterstain (blue) helps define the layers: granule (G), Purkinje (P), and molecular (M).

References:

1. Dingledine, R. et al. (1999) Pharmacol. Rev. 51, 7.

2. Song, I. and Huganir, R.L. (2002) Trends Neurosci. 25, 578.

3. Malinow, R. and Malenka, R.C. (2002) Annu. Rev. Neurosci. 25, 103.

AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each subfamily.

 

The a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members, AMPA1-AMPA4, that are also known as GluR1-GluR4, respectively.

 

The functional AMPA channel is believed to be a tetramer. Most neuronal AMPA receptors are heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers are also found.

 

AMPA receptors are permeable to Na⁺, K⁺, and Ca²⁺ cations. Permeability to Ca²⁺ is dependent upon the presence of AMPA2: whenever this subunit is present, the channel is impermeable to Ca²⁺. Ca²⁺ permeability of the AMPA2 receptor subunit is determined by the presence of arginine (R), as a result of RNA editing, at a critical site in the pore loop that is occupied by glutamine (Q) in the other AMPA subunits. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors are impermeable to Ca²⁺.

 

Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that open only in the presence of both glutamate and a depolarized membrane.

 

Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induces changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning.

 

Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.

 

Alomone Labs offers a highly specific antibody directed against an extracellular epitope of AMPA Receptor 2. The Anti-AMPA Receptor 2 (GluR2) (extracellular) antibody  (#AGC-005) can be used in immunohistochemical and Western blot applications and recognizes AMPA Receptor 2 from rat, mouse, and human samples.

 

Alomone Labs is pleased to offer a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. The Anti-AMPA Receptor 2 (GluR2) (extracellular)-ATTO-448 antibody (#AGC-005-AG) has been tested in immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.

 

 

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