Anti-ABCA5 Antibody

Anti-ABCA5 Antibody LS-B3554

ABCA5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3554 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3554 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

ABCA5 Antibody Details

 

 

Target:

Human ABCA5

 

Host:

Rabbit

 

Reactivity:

Human

 

Clonality:

IgG Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB)

 

Optimal dilution to be determined by the researcher.

 

ABCA5 Antibody Specificity

 

Immunogen:

ABCA5 antibody was raised against synthetic peptide from C-terminus of human ABCA5 (NP_061142.2, Q8WWZ7). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (92%); Dog, Rabbit (85%).

 

Epitope:

C-Terminus

 

Specificity:

Human ABCA5

ABCA5 Antibody Packaging

 

Presentation:

Lyophilized from PBS with 2% sucrose

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.