Anti-A3 Adenosine Receptor
- 貨號 Cat. No.
- AAR-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-A3 Adenosine Receptor
| Product#: | AAR-004 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)KETG AFYGR EFKTA K, corresponding to amino acid residues of 216-230 of human A3AR (Accession P33765). |
 |
| Putative epitope location: |
| 3rd intracellular loop. |
| Homology: |
Rat and mouse ?14/16 amino acid residues identical. |
| Reactivity Confirmed: |
| Human, rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of Malme-3M (human malignant melanoma) cell lysate. 1. Anti-A3AR antibody (#AAR-004), (1:200). 2. Anti-A3AR antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of A3 Adenosine Receptor in rat lung |
 |
Immunohistochemical staining of paraffin-embedded section of rat lung using Anti-A3AR antibody (#AAR-004), (1:50). Staining is specific for respiratory epithelial cells of the bronchiole (blue arrows) and pneumonocytes in the alveolar wall (a). Hematoxylin is used as the counterstain. |
| Immunocytochemistry: |
| Expression of A3 Adenosine Receptor in a human melanoma cell line |
 |
Immunocytochemical staining of A3AR in human melanoma cells (A2058). Paraformaldehyde-fixed and permeabilized human melanoma A2058 cells were stained as described. A, D. Immunocytochemical staining using Anti-A3AR antibody (#AAR-004), (1:100) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E. |
Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4
However, all are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3
Expression of A3AR has been reported in brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human A3 Adenosine receptor (A3AR). The Anti-A3AR antibody (#AAR-004) can be used in Western blot analyses, as well as immunocytochemical and immunohistocemical applications and should recognize A3AR from human, rat, and mouse samples.