Anti-a2B-Adrenoceptor (extracellular)
- 貨號 Cat. No.
- AAR-021
- 規格 Size
- 50 µl 0.2 ml
Description
Anti-a2B-Adrenoceptor (extracellular)
| Product#: | AAR-021 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
Rabbit. Type: |
| Epitope: |
Peptide (C)RPEPRGLPQ*SELNQE, corresponding to amino acid residues 160-174 of rat a2B- Adrenoceptor with replacement of cysteine 169 (C169) with serine (*S) (Accession P19328). |
 |
| Putative epitope location: |
| 2nd Extracellular loop. |
| Homology: |
| Rat - 14/15 amino cid residues identical; mouse - 13/15 amino acid residues identical; human - 10/14 amino acid residues identical. |
| Reactivity Confirmed: |
Rat. |
Applications:
| Western Blotting: |
|
 | Western blot analysis of rat kidney (lanes 1 and 5), lung (lanes 2 and 6), liver (lanes 3 and 7) lysates and rat skeletal muscle membranes (lanes 4 and 8): 1. Anti-a2B-Adrenoceptor (extracellular) antibody (#AAR-021), (1:200). 2. Anti-a2B-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of a2B-Adrenoceptor in rat lung
|
 |
Immunohistochemical staining of rat lung paraffin embedded sections using Anti-a2B-Adrenoceptor (extracellular) antibody (#AAR-021), (1:100). a2B-Adrenoceptor is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain. |
Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.
The Adrenoceptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: a1, a2 and b-Adrenoceptors. Each type is further divided into at least three subtypes: a1A, a1B, a1D, a2A, a2B, a2C, b1, b2, b31,2. The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2.
The a2B-Adrenoceptor has a distinct pattern of expression within the brain, liver lung and kidney, and recent studies using the knock out mouse system have shown that disruption of this receptor indeed affects mouse viability3, blood pressure responses to a2-Adrenoceptors agonists3 and the hypertensive response to salt loading4.
Like the a2A-Adrenoceptor subtype, the a2B-Adrenoceptor undergoes short term agonist promoted desensitization5. This desensitization is due to the phosphorylation of the receptor by G-protein coupled receptor kinases (GRKs)6 which ultimately promotes uncoupling of the receptor from the G-protein subunit7.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat a2B-Adrenoceptor. The Anti-a2B-Adrenoceptor (extracellular) antibody (#AAR-021) can be used for Western blot analysis. It has been designed to recognize a2B-Adrenoceptor from mouse, rat and human samples.