Anti-A2B Adenosine Receptor (extracellular)
- 貨號 Cat. No.
- AAR-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-A2B Adenosine Receptor (extracellular)
| Product#: | AAR-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide KDSAT NN*STE PWDGT TNESC, corresponding to amino acid residues 147-166 of human A2B-Adenosine Receptor with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). |
 |
| Putative epitope location: |
| Homology: |
| Rat ?14/19 amino acid residues identical; Mouse - 13/19 amino acid residues identical. |
| Reactivity Confirmed: |
Human, rat, and mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of A2B-Adenosine Receptor in lysates of human HL-60 (lanes 1 and 2) cells, rat brain (lanes 3 and 4), and mouse brain (lanes 5 and 6): 1, 3, 5. Anti-A2B-Adenosine Receptor (extracellular) antibody (#AAR-003), (1:200). 2, 4, 6. Anti-A2B-Adenosine Receptor (extracellular) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of A2B Adenosine Receptor in rat lung |
 |
Immunohistochemical staining of A2B Adenosine receptor in rat lung paraffin embedded sections using Anti-A2B Adenosine receptor antibody (#AAR-003) (1:50). Note that staining is present in the respiratory epithelium of the bronchiole (Br) as well as in the pneumonocytes of the alveolar wall (alveoli, (A)). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Counterstain is Hematoxilin. |
| References: |
| 1. Okusa, M.D. (2002) Am. J. Physiol. Renal Physiol. 282, F10. |
| 2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527. |
| 3. Nakata, H. (1989) J. Biol. Chem. 264, 16545. |
| 4. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13,3467. |
| 5. Polosa, R. (2002) Eur. Respir. J. 20, 488. |
Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors):
A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important in binding G proteins.1-3
The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4
The A2 Adenosine receptor subtype was subdivided according to affinity for adenosine; A2AAR has high affinity and A2BAR has low affinity.
A2BAR is expressed in mast cells, neutrophils, monocytes, macrophages, dendritic cells, T cells, bronchial epithelium, intestinal epithelium, smooth muscle, brain, and other cells.5 In intestinal epithelia, A2BAR was found to be the major adenosine receptor subtype expressed and is thought to be involved in diarrheal diseases. It is also believed to be involved in the pathogenesis of chronic airway inflammatory diseases.4 Thus, antagonists of A2BAR might be effective for the treatment of inflammatory gastrointestinal tract disorders4 as well as asthmaand chronic obstructive pulmonary disease.5
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human A2B Adenosine receptor (A2BAR). The Anti-A2B-Adenosine Receptor (extracellular) antibody (#AAR-003)) can be used for Western Blot analysis and Immunohistochemical applications. It has been designed to recognize A2B-Adenosine Receptor from human, rat and mouse samples.