Anti-A2A Adenosine Receptor
- 貨號 Cat. No.
- AAR-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-A2A Adenosine Receptor
| Product#: | AAR-002 |
| Sizes: |
50 µl |
0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)RQLKQMESQPLPGER corresponding to amino acid residues 201-215 of mouse A2A Adenosine receptor (Accession Q60613). |
| Putative epitope location: |
| 3rd intracellular loop. |
 |
| Homology: |
| Rat, human, and canis ?identical. |
| Reactivity Confirmed: |
| Rat, human. |
Applications:
| Western Blotting: |
 | Western blot analysis of A2A Adenosine Receptor in RAEC (rat aortic endothelial cells) (lanes 1 and 3), rat brain (lanes 2 and 4) and Jurkat (T lymphocyte, acute T cell leukemia) (lanes 5 and 6) lysates: 1,2,5. Anti-A2A Adenosine Receptor antibody (#AAR-002), (1:200). 3,4,6. Anti-A2A Adenosine Receptor antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of A2A Adenosine Receptor in rat diagonal band |
 |
Immunohistochemical staining of A2AAR using Anti- A2A Adenosine Receptor antibody (#AAR-002) in rat diagonal band. A2A Adenosine Receptor expression (green) appeared in the of broca in individual neurons (arrows) and in neuropil (asterisk). Hematoxylin is used as the counterstain. Expression of A2A Adenosine Receptor in rat lung |
 |
Immunohistochemical staining of rat lung paraffin emedded section using Anti-A2A Adenosine Receptor antibody (#AAR-002) (1:50). A2A Adenosine Receptor is expressed in the respiratory epithelium of the bronchioli (Br). Note that smooth muscle and endothelium in blood vessels are negative. Counterstain is Hematoxilin. |
| References: |
| 1. Okusa, M.D. (2002) Am. J. Physiol. Renal. Physiol. 282, F10. |
| 2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527. |
| 3. Nakata, H. (1989) J. Biol. Chem. 264, 16545. |
| 4. Cunha, R.A. (2001) Neurochem. Int. 38, 107. |
| 5. Fredholm, B.B. et al. (2000) Naunyn-Schmiedebergs Arch. Pharmacol. 362, 364. |
| 6. Canals, M. et al. (2005) J. Neurochem. 92, 337. |
| 7. Ohta, A. and Sitkovsky, M. (2001) Nature 414, 916. |
| 8. Ohta, A. et al. (2006) Proc. Natl. Acad. Sci. 103, 13132. |
Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions of many tissues including brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily, which share a common structure of seven putative transmembrane domains, an extracellular NH2 terminus, cytoplasmic COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3
Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS.5,6
A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues.8
Alomone Labs is pleased to introduce a new antibody directed against the A2A-Adenosine receptor, Anti-A2A Adenosine Receptor antibody (#AAR-002). This antibody recognizes human, rat, and mouse A2AAR.