Anti-a1B-Adrenoceptor (extracellular)-ATTO-488

 

Anti-a_1B-Adrenoceptor (extracellular)-ATTO-488

Product#:

AAR-018-AG

Sizes:

50 µl

Host:

Rabbit. Type: Polyclonal.

Epitope:

Peptide (C)KNANFTGPNQTSSNS corresponding to amino acid residues 21-35 of human a1B-adrenoceptor (Accession P35368).

Putative epitope location:

Extracellular, N-terminus.

Homology:

Mouse-14/15 amino acid residues identical ; rat ?3/15 amino acid residues identical.     Label: ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Western blot analysis of a1B-adrenoreceptor using unlabeled Anti-a1B-adrenoreceptor (extracellular) antibody (#AAR-018) in lysates of: rat brain (lanes 1and  3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6). 1,2,5. Anti- a1B-adrenoreceptor (extracellular) antibody (#AAR-018), (1:200). 3,4,6. Anti-a1B-adrenoreceptor (extracellular) antibody, preincubated with the control peptide antigen.

Immunocytochemistry:

Expression of a1B-adrenoreceptor in GH3 cells

Immunocytochemical staining of GH3 cells with Anti-a1B-adrenoreceptor (extracellular)-ATTO-488 antibody (#AAR-018-AG) (green). Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342 (blue).

Flow Cytometry:

Flow cytometry analysis of living GH3 cells ______ Unstained cells. ______ Anti-Anti-a1B-adrenoreceptor (extracellular)-ATTO-488 antibody (#AAR-018-AG) (10ç—¢/5x105 cells).

Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.

 

The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: a₁, a₂ and b adrenoceptors. Each type is further divided into at least three subtypes: a_1A, a_1B, a_1D, a_2A, a_2B, a_2C, b₁, b₂, b₃. ^1,2

They are expressed in nearly all peripheral tissues and in the central nervous system.^1,2

 

All a₁-Adrenoceptors (a₁-ARs) activate phospholipases C and A2.³

In addition to mobilizing intracellular calcium, the a₁-ARs have also been shown to activate calcium influx via voltage-dependent and independent calcium channels.⁴

 

The a_1B- Adrenoceptor shows the highest levels in the spleen, kidney, cerebellum,

and fetal brain.⁵

a_1B- Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.⁵

 

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human a_1B- Adrenoceptor. The Anti- a_1B^- Adrenoceptor (extracellular) (#AAR-018) can be used for Western blotting, Immunocytochemistry and Indirect Flow Cytometry applications. It has been designed to recognize a_1B- Adrenoceptor from human, rat and mouse samples.

 

Alomone Labs is offering a new version of this antibody that is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti- a_1B^- Adrenoceptor (extracellular)-ATTO-448 antibody (#AAR-018-AG) has been tested in immunocytochemical and flow cytometry applications and is specially suited for experiments requiring simultaneous labeling of different markers.