Angiotensin I Converting Enzyme Activity (ACE1) Assay Kit (Fluorometric)

Alternate Name :

Tag Line :

Industry's first kit to detect ACE1 activity in biological samples

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method - Fluorescence (Ex/Em = 330/430 nm)
• Applications- Detection of ACE1 activity in tissue/cell lysates

Detection Method: Fluorometric (Ex/Em = 330/430 nm)

Features & Benefits:

• Simple one-step reaction
• Takes only 1-2 hrs
• Non-radiometric fluorescent detection
• HTP adaptable

Description:

Angiotensin I converting enzyme (ACE1, EC: 3.4.15.1), a dipeptidyl carboxypeptidase, is part of the renin-angiotensin system (RAS) that controls regulation of blood pressure by cleaving the C-terminal dipeptides of angiotensin I and bradykinin. It is found on the luminal surface of vascular endothelial cells, especially in pulmonary tissues. Elevated levels of ACE1 are found in patients suffering from sarcoidosis, leprosy, hyperthyroidism, acute hepatitis, primary biliary cirrhosis, diabetes mellitus, multiple myeloma, osteoarthritis, amyloidosis, Gaucher’s disease, pneumoconiosis, histoplasmosis and miliary tuberculosis. BioVision’s ACE1 Activity Assay Kit utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples