Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K677-100
- 規格 Size
- K677-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method-Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 30 minutes to complete the assay
• Fast and convenient
• Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples
Description:
α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. BioVision's α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.