Alpha Galactosidase (α-Gal) Activity Assay Kit (Fluorometric)

Alternate Name :

Tag Line :

The most sensitive assay to measure α-Gal activity

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method: Fluorescence (Ex/Em = 360/445 nm)
• Application:
Measurement of α-Galactosidase activity in various samples

Detection Method: Fluorescence (Ex/Em = 360/445 nm)

Features & Benefits:

• Quick and easy method to measure Alpha Galactosidase activity.
• Detection limit: < 0.1 µU.
• Specific: does not detect beta galactosidase activity

Description:

Alpha-Galactosidase (α-Gal; EC 3.2.1.22) hydrolyzes alpha-galactosyl moieties found in glycolipids and glycoproteins. In mammals, α-Gal hydrolyzes poly- and oligosaccharides commonly found in dietary sources that are difficult to digest. Therefore, α-Gal is used in dietary supplements that help to reduce the production of intestinal gases due to consumption of certain foods. It is known total α-Gal activity is due to two major isozymes with unique, yet different thermostability profiles. Alpha-Galactosidase A, is thermolabile and represents approximately 90% of total α-Gal activity. Fabry Disease, a lysosomal disease disorder, is characterized by mutations in alpha-Galactosidase A. These mutations cause abnormal accumulation of glycosphingolipids in lysosomes. BioVision’s Alpha Galactosidase Activity Assay Kit provides a simple, rapid way to monitor total α-Gal activity in wide variety of biological samples. In this kit, α-Gal cleaves a synthetic specific substrate releasing a fluorophore, which can be easily quantified (Ex/Em= 360/445 nm). The assay is specific, sensitive and can detect as low as 0.1 µU of α-Galactosidase activity.