Albumin-to-Creatinine Ratio (ACR)

Alternate Name :

Tag Line :

Only kit on the market to measure UACR

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em = 600/630 nm) & Absorbance (OD 570 nm)
• Applications- Estimation of albumin in biological samples
- Estimation of creatinine in biological samples
- Determination of UACR in mammalian urine samples

Detection Method: Fluorescence (Ex/Em = 600/630 nm) & Absorbance (OD 570 nm)

Features & Benefits:

• Simple & Sensitive
• High-Throughput adaptable
• Suitable for detecting albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio (UACR)

Description:

Urine Albumin-to-Creatinine Ratio (UACR) is one of the two markers used to determine chronic kidney disease (CKD). UACR is recommended to be measured on regular basis on people living with Type I and Type II diabetes. UACR is defined as the ratio between albumin (reported in mg/dl) and creatinine (reported in g/dl). This ratio estimates the amount of albumin excreted in urine during a 24 hr period. Albuminuria is diagnosed when UACR is greater than 30 mg albumin/g creatinine. BioVision’s UACR Assay Kit provides a simple, sensitive, and high-throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/ml), creatinine (detection range: 0.002 -0.5 mg/ml) and urine albumin-to-creatinine ratio. The UACR ratio is determined in two steps: First, albumin is determined by using a probe (AB580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Sarcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.