Proteins and Peptides
AKT3 Antibody (LS-B1319)
- 貨號 Cat. No.
- LS-B1319
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B1319 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C497. It has been validated for use in the following assays: IHC. |
| Antibody Target: | V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) |
| Synonyms: | AKT3, akt3, AKT3/PKBgamma, PKB gamma, pkbg, prkbg, protein kinase B gamma , rac-gamma, RAC-PK-gamma, serine/threonine kinase rac-gamma, STK-2, v-akt murine thymoma viral oncogene homolog 3 |
| Family: | Protein Kinase |
| Subfamily: | RAC/AKT |
| Host: | AKT3 antibody was produced in Mouse |
| Antigen Species: | AKT3 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antibody Clone Name: | 66C1247.1 |
| Antigen Type: | Synthetic peptide - KLH conjugated |
| Immunogen: | AKT3 antibody was raised against synthetic peptide |
| Antibody Specificity: | A synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human Akt3, GenBank no. gi|4574744|gb|AAD24196.1|AF135794_1. This sequence is identical in human, mouse, rat, cow, dog, and chicken. |
| Antigen Modification: | aa119-136 |
| Predicted Species Reactivity: | Mouse, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, 0.05% BSA, 0.05% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B1319 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1319 was determined to be 10 ug/ml. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 - 2 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.5 mg/ml |


