AKT1S1 / PRAS40 Antibody (LS-B2541)

貨號 Cat. No.: LS-B2541
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2541
Antibody Validation:	This antibody replaces catalog number LS-C17345. It has been validated for use in the following assays: IHC-P.
Antibody Target:	AKT1S1 / PRAS40
Synonyms:	AKT1S1 Antibody, Lobe Antibody, MGC2865 Antibody, PRAS40 Antibody
Host:	AKT1S1 antibody was produced in Mouse
Antigen Species:	AKT1S1 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1,k
Antigen Type:	Recombinant protein
Immunogen:	AKT1S1 antibody was raised against recombinant full length human PRAS40 expressed in E. coli.
Antibody Specificity:	Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
Purification:	Protein A/G Column
Presentation:	PBS, pH 7.2, 0.1% sodium azide, 1% BSA.
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (0.1 - 1 µg/ml), Immunoprecipitation (IP) (Optimal dilution to be determined by the researcher)
Size:	50 µg