AKT1 Antibody (LS-B1183)

貨號 Cat. No.: LS-B1183
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B1183
Antibody Validation:	This antibody replaces catalog number LS-C18880. It has been validated for use in the following assays: IHC.
Antibody Target:	v-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Synonyms:	AKT1, akt, akt-1, akt/pkb, akt1, C-AKT, pkb, PKBalpha, PRKBA, protein kinase B, protein kinase B alpha, RAC-PK-alpha, related to A and C kinase rac-alpha, v-akt murine thymoma viral oncogene homolog 1
Family:	Protein Kinase
Subfamily:	RAC/AKT
Host:	AKT1 antibody was produced in Rabbit
Antigen Species:	AKT1 antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	AKT1 antibody was raised against synthetic peptide
Antibody Specificity:	The C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Antigen Modification:	pSer473
Purification:	Immunoaffinity Chromatography
Presentation:	0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:200 - 1:1000), ELISA (1:15000 - 1:60000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml