AKT (AKT1/AKT2/AKT3) Antibody (LS-B2844)

貨號 Cat. No.: LS-B2844
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2844
Antibody Validation:	This antibody replaces catalog number LS-C18882. It has been validated for use in the following assays: IHC-P.
Antibody Target:	AKT (AKT1/AKT2/AKT3)
Host:	Rabbit
Antigen Species:	Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide - KLH conjugated
Immunogen:	Synthetic peptide
Antibody Specificity:	Synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Antigen Modification:	C-Terminus
Purification:	Delipidation and Defibrination
Presentation:	0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Recommended Storage:	Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:500 - 1:2000), Immunoprecipitation (IP), Immunofluorescence (IF) (1:100 - 1:1000), ELISA (1:2000 - 1:10000) (Optimal dilution to be determined by the researcher)
Size:	50 µl