AHCY Inhibitor Screening Kit (Fluorometric)

Alternate Name : S-adenosylhomocysteine hydrolase (SAHH) Inhibitor Screening Kit (Fluorometric), S-adenosylhomocysteine synthase Inhibitor Screening Kit (Fluorometric), Adenosylhomocysteine hydrolase Inhibitor Screening Kit (Fluorometric)

Tag Line :

Simple assay for high-throughput screening of AHCY Inhibitors

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em = 392/482 nm)
• Application-Screening/characterizing/studying AHCY inhibitors

Detection Method: Fluorescence (Ex/Em = 392/482 nm)

Features & Benefits:

• Simple and reliable test to screen AHCY inhibitors
• High-throughput suitable
• Includes Inhibitor Control (3-Deazaneplanocin A)

Description:

Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In BioVision’s AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.