aFGF acidic protein

貨號 Cat. No.
aFGF acidic protein
規格 Size
50ug
品牌 Brand
Gene Research

Description




























































Product Name aFGF acidic protein
Product type Proteins and Peptides
Size 50ug
Peptide Recombinant full length protein, with a N-terminal hexahistidine

tag.
Tested applications SDS-PAGE, Western Blotting, Cell Test
Application notes Recommended dilutions

SDS-PAGE: Use at an assay dependent dilution

Western Blotting: Use at an assay dependent dilution

Optimal dilutions/concentrations should be determined by the end

user.
Relevance Fibroblast Growth Factor acidic (FGF-a or FGF-1) is a heparin

binding growth factor, which stimulates the proliferation of a wide

variety of cells including mesenchymal, neuroectodermal and

endothelial cells.
Storage buffer n/a
Purity >95% by SDS-PAGE and PLC analysis.
Form Lyophilised
Storage instructions Aliquot and store at -20°C. Avoid freeze / thaw cycles.
Expression system Yeast (Eukaryotic cell)
Sequence Human FGF acidic is a 15.8 kDa protein containing 141 amino acid

residues:

MFNLPPGNYK KPKLLYCSQG GHFLRILPDG

TVDGTRDRSD QHIQLQLSAE SVGEVYIKST

ETGQYLAMDT DGLLYGSQTP NEECLFLERL

EENHYNTYIS KKHAEKNWFV GLKKNGSCKR

GPRTHYGQKA ILFLPLPVSS D
 































0.01 ug/ml


1 ug/ml


10 ug/ml


100 ug/ml

Fig 1: aFGF Treatment of 3T3 Cell Line

Different concentration of purified aFGF was added into 3T3 cell culture medium.

Pictures were taken after 24 or 48 hours.

The purified growth factor increased the proliferation of 3T3 cell line.

生長因子在3T3的細胞中用不同濃度培養,於24或48小時拍照觀察

生長因子可增加3T3分化生長的能力aFGF

















Fig 2: Affinity Purification aFGF

protein was purified by Fast

Flow affinity column.



aFGF蛋白質經由Fast Flow親合

性管柱純化




Fig 3:(a) Western Blotting of aFGF

The blotting membrane was hybridized

with anti-aFGF antibody to check the purity.

利用aFGF的抗體來檢查轉漬膜
(b)SDS Page of aFGF

SDS page was used to check the

aFGF molecular weight and purity.

使用SDSPage來檢測aFGF的分子

量大小及純度



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