AFC Standard

貨號 Cat. No.
B-210
規格 Size
100 μl
品牌 Brand
Boston Biochem

Description

AFC Standard

Catalog #: B-210




AFC Standard



A fluorogenic standard useful for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin (AFC) release such as those utilizing ubiquitin-AFC (U-551). The concentration of the AFC released can be calculated from a standard curve prepared with AFC standards.


Product Information



Quantity: 

100 μl






Stock: 

100μl of 50μM AFC in DMSO




 


Use & Storage



Use: 

Serial dilute in reaction buffer to form a standard curve within the range of sensitivity for specific plate reader.

Recommend dilution series starting at 500nM AFC

The excitation and emission wavelengths of AFC are A400nm and A505nm

respectively.






Storage: 

Store at -20°C. Avoid multiple freeze/thaw cycles.



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