Proteins and Peptides
ADORA2A Antibody (LS-B2122)
- 貨號 Cat. No.
- LS-B2122
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2122 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C20307. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Adenosine A2a Receptor (ADORA2A) |
| Synonyms: | ADORA2A, a2a receptor, adenosine a2a receptor, adora2, adora2a, rdc8 |
| Family: | GPCR |
| Subfamily: | Adenosine |
| Host: | ADORA2A antibody was produced in Mouse |
| Antigen Species: | ADORA2A antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG2a |
| Antigen Type: | Recombinant protein |
| Immunogen: | ADORA2A antibody was raised against 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. |
| Antibody Specificity: | Recognizes human adenosine receptor A2A, Mr 43kD. Species cross-reactivity: Mouse, rat, rabbit and canine. Cross-reactivity with guinea pig also reported. Species cross-reactivity: Does not recognize Adenosine Receptor A2A in knockout mice. |
| Antigen Modification: | 3rd Intracellular Domain |
| Predicted Species Reactivity: | Dog, Guinea pig, Mouse, Rabbit, Rat |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.0, 0.01M EDTA |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2122 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2122 was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - 4% paraformaldehyde (IHC-Pfa) (1 µg/ml), Western blot (WB) (1:1000), Flow Cytometry (Flo) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 1 mg/ml |
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