ActiveMax® Human G-CSF Protein, Tag Free

Source : 

ActiveMax® Human G-CSF, Tag Free (GCF-H5214) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Pro 204 (Accession # NP_757373.1).

 

Molecule : G-CSF

 

Synonyms : G-CSF,CSF3,C17orf33,CSF3OS,MGC45931

 

Format : Powder

 

Category : ActiveMax® Cytokines

 

Accession : NP_757373.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 18.7 kDa

 

Characteristics :

This protein carries no "tag". The protein has a calculated MW of 18.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.

 

References :

(1) Schneider A,, et al., 2005, J. Clin. Invest. 115 (8): 2083–98.
(2) Pitzer C, et al., 2008, Brain 131 (Pt 12): 3335–47.