Active Rec. HAT

SOURCE:

The recombinant P/CAF (p300/CBP-associated factor) encoding human cDNA sequence from 352 to 658 of gene accession number NM003804 was produced from E. coli and then purified by several chromatographic steps.

FORM: Lyophilized

PURITY: >95% by SDS-PAGE

STORAGE CONDITIONS:

Store at –70^oC, stable for at least 1 year as supplied. Avoid freeze/thaw cycles.

RECONSTITUTION:

Reconstitute in MilliQ water to a concentration of 1 mg/ml. The solution can then be used in bioassay, or store at -20^oC for future use.

APPLICATIONS:

Recombinant P/CAF is useful in HAT inhibitor screening, kinetic studies, and HAT substrate cleavages in vitro or in tissue culture. P/CAF can also be used as positive control in HAT activity assay (BioVision, Cat# K332-100).

ACTIVITY:

Activity of P/CAP was routinely tested for its ability to cleave HAT Substrate. A detailed assay procedure is available (BioVision, Cat.# K332-100).

FOR RESEARCH USE ONLY! Not to be used in human.