Activated Protein C (APC) Inhibitor Screening Kit (Colorimetric)

Alternate Name :

Tag Line :

High-throughput compatible, quick and easy screening for Activated Protein C ligands; Includes human recombinant APC

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method - Colorimetric (OD 405 nm)
• Species reactivity - N/A
• Applications - Screening of drugs and novel ligands for interaction with human recombinant Activated Protein C

Detection Method: Detection method - Colorimetric (OD 405 nm)

Features & Benefits:

• Simple, highly sensitive, high-throughput compatible
• Rapid screening of Activated Protein C ligands
• Kit includes PPACK (APC inhibitor) and a stable, recombinant human APC

Description:

Activated protein C (APC) (3.4.21.69), a Vitamin K dependent serine protease, is formed by activation of Protein C by Thrombomodulin bound on the surface of endothelial cells. By degrading coagulation factors Va and VIIIa, APC inhibits blood coagulation which helps in the prevention of thrombosis. Blood coagulation can be stimulated by inhibition of APC. Thus, APC inhibitors play an important role in the treatment of disease like Haemophilia. BioVision’s APC Inhibitor Screening Kit can be used to screen for potent inhibitors of APC activity. It utilizes the proteolytic activity of an active APC on a peptide substrate which releases a colored product. The released product can be easily quantified using an absorbance microplate reader. In the presence of an APC inhibitor, the enzyme decreases/loses activity resulting in lower/no absorbance. This assay kit is simple and can be used to identify and characterize APC inhibitors in a high-throughput format. As a control, we provide the inhibitor PPACK dihydrochloride - a known APC inhibitor.