Proteins and Peptides
ACTA2 Antibody (LS-B2506)
- 貨號 Cat. No.
- LS-B2506
- 規格 Size
- 50 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2506 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C34848. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Actin, Aortic Smooth Muscle (ACTA2) |
| Synonyms: | ACTA2, AAT6, actin, aortic smooth muscle, ACTSA, ACTVS, Alpha smooth muscle Actin, Alpha-actin-2, alpha-cardiac actin , Alpha-cardiac actin |
| Host: | ACTA2 antibody was produced in Rabbit |
| Antigen Species: | ACTA2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Antigen Type: | Synthetic peptide |
| Immunogen: | ACTA2 antibody was raised against a synthetic peptide from N-terminus of human alpha smooth muscle isoform of actin. Cellular Localization: Cytoplasmic |
| Antibody Specificity: | Recognizes smooth muscle Actin. Reacts with tumors arising from smooth muscles and myoepithelial cells. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Species cross-reactivity: Human, baboon, bovine, rabbit, mouse, rat and chicken. |
| Antigen Modification: | N-Terminus |
| Predicted Species Reactivity: | Bovine, Chicken, Mouse, Rabbit, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | 10 mM PBS, pH 7.6, 0.2% BSA, 15 mM sodium azide. |
| Recommended Storage: | Long term: -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2506 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2506 was determined to be 5 ug/ml. Positive control: Smooth muscle of arteries, gut wall, uterus and leiomyosarcoma. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µl |
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