Proteins and Peptides
ACTA2 Antibody (LS-B2161)
- 貨號 Cat. No.
- LS-B2161
- 規格 Size
- 250 µl
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2161 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C34841. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Actin, Aortic Smooth Muscle (ACTA2) |
| Synonyms: | ACTA2, actin, aortic smooth muscle, alpha-cardiac actin |
| Host: | ACTA2 antibody was produced in Rabbit |
| Antigen Species: | ACTA2 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | ACTA2 antibody was raised against synthetic peptide corresponding to N-terminus of human smooth muscle alpha actin. Cellular Location: Cytoplasmic. |
| Antibody Specificity: | Recognizes smooth muscle cells in vessel walls, gut wall and myometrium. Recognizes myoepithelial cells in breast and salivary gland. Recognizes tumors arising from smooth muscles and myoepithelial cells. Does not cross-react with the other actins, such as beta- and gamma-cytoplasmic, striated muscle, and myocardium. Species cross-reactivity: Chicken, bovine, human, mouse, baboon, rabbit and rat. |
| Antigen Modification: | N-Terminus |
| Predicted Species Reactivity: | Bovine, Chicken, Mouse, Rabbit, Rat |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, pH 7.4, 0.09% sodium azide. |
| Recommended Storage: | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2161 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2161 was determined to be 5 ug/ml. Western Blot: 0.5-2 ug/ml. Predicted MW: 42kD. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 2 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 250 µl |
| Concentration: | 0.2 mg/ml |
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