ACT1 Polyclonal Ab
- 貨號 Cat. No.
- 3403-100
- 規格 Size
- 100 ug
Description
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pbHOST:/b Rabbit/p
pbIMMUNOGEN:/b Synthetic peptide of human ACT1/p
pbSPECIES REACTIVITY: /bhuman/p
pbFORMULATION: /b100 ug (0.5 mg/ml) affinity purified rabbit anti-ACT1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal./p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bAct1 (NF-?B activator 1) contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- ?B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF-?B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes./p
pbSPECIFICITY: /bThe antibody detects 60 kDa A ct1 from human samples./p
pbAPPLICATION AND USAGE: /bThe antibody can be used for Western blotting (1-4 ug/ml). However, the optimal conditions should be determined individually. Human breast cancer cell line MCF-7 can be used as a positive control./p
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pbHOST:/b Rabbit/p
pbIMMUNOGEN:/b Synthetic peptide of human ACT1/p
pbSPECIES REACTIVITY: /bhuman/p
pbFORMULATION: /b100 ug (0.5 mg/ml) affinity purified rabbit anti-ACT1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal./p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long-term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bAct1 (NF-?B activator 1) contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- ?B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF-?B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes./p
pbSPECIFICITY: /bThe antibody detects 60 kDa A ct1 from human samples./p
pbAPPLICATION AND USAGE: /bThe antibody can be used for Western blotting (1-4 ug/ml). However, the optimal conditions should be determined individually. Human breast cancer cell line MCF-7 can be used as a positive control./p
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