ACT I
- 貨號 Cat. No.
- CLC003
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC003) | |
Designations | ACT I |
| Cell type | Hybridoma |
Growth property | Suspension |
| Seeding density(cells/ml) | 2X106 |
Survival rate | 95% |
Culture medium | 80%RPMI medium 1640 with HAT (containing:hypoxanthine,10-4M) aminopterin 4.0x10-7M thymidine 1.6x10-5M)+20% fetal bovine serum |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 95% culture medium+5% DMSO |
Medium renewal | every 2 to 3 days |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Cellular products | Anti-Actin |
Comments | Alternatively cultures can be established by centrifugation with subsequent resuspension at 105 viable cells/ml Maintain cell density between 105 and 106 per ml |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found,please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.