Aconitase Activity Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K716-100
- 規格 Size
- K716-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 etc.) 、 fermentation media 、 Food samples 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay, HTS
Storage Conditions: 4°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (450 nm)
• Species reactivity- Mammalian
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ less than 40 minutes
• Fast and convenient
• Sensitive assays for measuring Aconitase activity in various biological samples.
Description:
Aconitase (aconitate hydratase; EC 4.2.1.3) is an iron-sulfur protein containing an [Fe₄S₄]²ᶧ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Tissue contains two aconitases, a mitochondrial (m-) and a cytosolic (c-) aconitase. They are related, but distinctly different enzymes and are coded for on different chromosomes. Loss of aconitase activity in cells or other biological samples treated with pro-oxidants has been interpreted as a measure of oxidative damage. BioVision’s Aconitase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring Aconitase activity in biological samples. In the assay, citrate is converted by aconitase into isocitrate, which is further processed resulting in a product that converts a nearly colorless probe into an intensely colored form with a λmax at 450nm.