Acid Sphingomyelinase Assay Kit II (Colorimetric)
Kits
- 貨號 Cat. No.
- K192-100
- 規格 Size
- K192-100
- Reactivity
- All
- Sample Type
- Cell lysate 、 Serum 、 Tissue lysate
Description
Alternate Name : Acid Sphingomyelinase,SMase,Sphingomyelinase
Tag Line :
Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples
Storage Conditions: -80°C
Shelf life: 12 months
Summary :
Detection Method: Colorimetric (OD= 570 nm)
Features & Benefits:
Complete set of reagents for the detection of Acid SMase in biological samples,Simple, specific (detect acid SMase activity only)
Description:
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.