Acid Phosphatase Activity Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K411-500
- 規格 Size
- K411-500
- Sample Type
- as well as many other biological fluids 、 Cell and tissue lysates 、 culture media 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Highly Sensitive Assay, HTS
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (405 nm)
• Species reactivity- Mammalian
• Applications- Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Detection Method:
Features & Benefits:
• The assay detects acid phosphatase (AP) activity conveniently in various biological samples
Description:
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. BioVision’s Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples.