Acetoacetate Colorimetric Assay Kit

Alternate Name :

Tag Line :

Only kit on the market to measure acetoacetate

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (550 nm)
• Applications: - Measurement of acetoacetate in various biological samples
• Analysis of acetoacetate in pathological conditions such as Type I diabetes, alcoholism, etc.

Detection Method: Absorbance (550 nm)

Features & Benefits:

• Specific for acetoacetate and does not detect 3-β-hydroxybutyrate
• The assay can detect less than 25 µM of acetoacetate in various sample types.

Description:

Acetoacetate (AcAc), a β-ketoacid, is one of the three ketone bodies and is formed via condensation of two molecules of acetyl-CoA in liver mitochondria. AcAc can be enzymatically reduced to 3-β-hydroxybutyrate (β-HB), or decarboxylated producing acetone (CH3)2CO). Ketone bodies (βHB: 78%; AcAc: 20% & CH3)2CO: 2%) are mainly used as an alternative energy source when glucose cannot be delivered to the system. Excessive concentration of ketone bodies (ketoacidosis) is observed in patients with Type I diabetes, severe starvation or alcoholism. Traditionally, AcAc levels have been qualitatively detected using dipsticks that use sodium nitroferricyanide as a chromophore. BioVision’s Acetoacetate Assay Kit has adapted that principle with a modification that provides a sensitive method to quantitate endogenous levels of AcAc in human blood, and urine. In this non-enzymatic assay, AcAc reacts with a substrate to generate a colored product that can be measured at 550 nm. The reaction is specific for AcAc and does not detect 3-β-hydroxybutyrate. The assay kit can detect samples containing acetoacetate as low as 25 µM.