Proteins and Peptides
ABL1 Antibody (LS-B2776)
- 貨號 Cat. No.
- LS-B2776
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2776 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C12197. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | ABL1 |
| Synonyms: | ABL1 Antibody, Abl Antibody, Bcr-Abl Antibody, Bcr/abl Antibody, C Abl Antibody, c-abl Antibody, jtk7 Antibody, p150 Antibody, proto-oncogene tyrosine-protein kinase abl Antibody, v-abl Antibody |
| Family: | Protein Kinase |
| Subfamily: | Abl |
| Host: | ABL1 antibody was produced in Mouse |
| Antigen Species: | ABL1 antibody was raised against Human |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Antigen Type: | Recombinant protein |
| Immunogen: | ABL1 antibody was raised against recombinant Abl protein. |
| Antibody Specificity: | Recognizes human c-Abl p120 and chimeric Bcr/Abl proteins. Species cross-reactivity: mouse. |
| Predicted Species Reactivity: | Mouse |
| Purification: | Protein G Column |
| Presentation: | PBS, pH 7.4. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2776 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2776 was determined to be 10 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Western Blot: 1 ug/ml for 2hrs at RT. Positive control: HeLa cells or placenta. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml), Western blot (WB) (1 µg/ml), Immunoprecipitation (IP) (2 µg/mg lysate), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |


