A23187 (Calcimycin)
- 貨號 Cat. No.
- A-600
- 規格 Size
- 10x1mg 5mg 5x5mg
Description
A23187 (Calcimycin)
Widely Used Ca2+ Ionophore
| Product#: | A-600 |
| Sizes: |
| 10 x 1 mg |
| 5 mg |
| 5 x 5 mg |
| M.W.: | 523.6 daltons |
| CAS No.: | [52665-69-7] |
| Structure: |
| . |
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| Bioassay: |
| The biological activity was tested to confirm the ability of A23187 to induce apoptosis in Jurkat cells.
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Jurkat cells were grown to 70% confluency. A23187 (2 mM) or vehicle were added to cell culture for 6 hours. |
| References: |
1. | Berridge, M.J. (1993) Nature 361, 315. |
2. | Scharff, O. et al. (1993) Physiol. Rev. 73, 547. |
3. | Orrenius, S. et al. (2003) Nat. Rev. Mol. Cell Biol. 4, 552. |
4. | Hajnoczky, G. et al. (2003) Biochem. Biophys. Res. Commun. 304, 445. |
5. | Caron-Leslie, L.A. et al. (1991) Mol. Endocrinol. 5, 1169. |
6. | Caron-Leslie, L.A. et al. (1994) FASEB J. 8, 639. |
7. | Rzigalinski, B.A. et al. (1994) Biochim. Biophys. Acta 1223, 219. |
8. | Vrana, J.A. et al. (1998) Int. J. Oncol. 12, 927. |
9. | Petersen, A. et al. (2000) Brain Res. 857, 20. |
10. | Cheng, C.H. et al. (2002) FEBS Lett. 516, 191. |
11. | Jansen, S. et al. (2003) Biol. Trace Elem. Res. 96, 133. |
A23187 is a mobile-carrier Ca2+ ionophore (allows Ca2+ ions to cross cell membranes) originally isolated as an antibiotic from Streptomyces chartreusensis. It is used to increase intracellular Ca2+ levels, and for in vitro studies related to intracellular Ca2+ mediated events. A23187 can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.1,2
Furthermore, A23187 induces in a concentration and time-dependent manner, Ca2+ triggered cell apoptosis.3,4 This property was exploited to clarify apoptotic events in different cell lines such as mouse lymphoma cell line (S49),5,6 histiocytic lymphoma U937,7 human myeloid leukemia cells (HL-60),8 cultured striatal neurons,9 renal tubular cells,10 and C6 glioma cells.11