6-keto-PGF1 α ELISA Kit

Alternate Name :

Tag Line :

A Competitive ELISA kit for in vitro quantitative determination of 6-keto-PGF1α concentrations in serum, plasma and other biological fluids.

Storage Conditions: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Sensitivity: 9.38 pg/mL, Precision: Coefficient of variation is < 10%., Detection Range: 15.63-1000 pg/mL, Specificity: This kit recognizes 6-keto-PGF1a in samples. No Significant cross-reactivity or interference between 6-keto-PGF1a and analogues was observed.

Description:

6-keto-PGF1α is a stable hydrolysis product of prostacyclin. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia. BioVision 6-keto-PGF1α ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with 6-keto-PGF1α. During the reaction, 6-keto-PGF1αin the samples or standard competes with 6-keto-PGF1α coated on the plate for binding to the anti-6-keto-PGF1αantibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of 6-keto-PGF1α. The concentration of 6-keto-PGF1αin the samples can be calculated by comparing the OD of the samples to the standard curve.