4-Hydroxynonenal (4-HNE) ELISA Kit

Alternate Name :

Tag Line :

A competitive ELISA kit for the quantitative measurement of 4-Hydroxynonenal in serum, plasma, tissue homogenates and other biological fluids

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of 4-Hydroxynonenal in serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of 4-Hydroxynonenal in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 31.25 - 2000 pg/ml
Sensitivity: < 18.75 pg/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
No significant cross-reactivity or interference was observed.

Description:

4-hydroxynonenal (4-HNE) is a lipid peroxidation product derived from oxidized ω-6 polyunsaturated fatty acids such as arachidonic acid. 4-HNE is widely used as a marker of lipid peroxidation. It is also in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction. It exhibits various biological activities such as cytotoxicity, growth inhibiting activity, genotoxicity, and chemotactic activity. It also inhibits Sirtuin 3 (SIRT3) via thiol-specific modification. BioVision’s 4-Hydroxynonenal ELISA kit is a competitive ELISA assay for the quantitative measurement of 4-HNE in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of 4-HNE captured from the samples.