4-HNE ELISA Kit

This 4-HNE ELISA kit is intended for laboratory research use only and not for
use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow
and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to
measure the concentration of 4-HNE in the sample, this 4-HNE ELISA Kit includes a set of
calibration standards. The calibration standards are assayed at the same time as the samples and
allow the operator to produce a standard curve of optical density versus 4-HNE concentration.
The concentration of the samples is then determined by comparing the O.D. of the samples to the
standard curve.