3-Nitrotyrosine ELISA Kit
Kits
- 貨號 Cat. No.
- K4158-100
- 規格 Size
- K4158-100
- Reactivity
- Universal
- Sample Type
- cell culture supernatant and other biological fluids. 、 Plasma 、 Serum 、 tissue homogenates
Description
Alternate Name : 3-Nitrotyrosine ELISA Kit
Tag Line :
An ELISA kit for the quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids
Storage Conditions: 4°C
Shelf life: 6 months
Summary :
• Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Method: Absorbance (450 nm)
Features & Benefits:
Easy, convenient and time-saving method to measure the level of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Description:
3-Nitrotyrosine (3-NT) is a biomarker of nitrogen free radical species modified proteins in systemic autoimmunogenic conditions. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.