Kits
| Article number: | KC3400 |
| Method: | HPLC |
| Tests: | 100 Tests |
| Matrix: | Serum |
| Volume: | 500 µl |
| Detection Elution: | UV |
| Standards: | isocratic |
| D-vitamins and calciferols arise from provitamins by the UV radiation of sunlight catalysed splitting of the B-ring of the steran backbone. The two most important D-vitamins are vitamin D3 and vitamin D2. In the contrary to vitamin D2 which has to be added via food, vitamin D3 can be produced in the liver. In the skin formed or together with vitamin D2 by food ingested vitamin D3 is bound to a vitamin D binding-protein in the plasma, transported into the liver and hydroxylated in position 25 to form 25-OH-D. More than 95% of 25-OH-D is 25-OH-D3. 25-OH-D2 is only detectable in patients with medication of vitamin D2. |