20S Proteasome
- 貨號 Cat. No.
- E-355
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
20S Proteasome
mouse
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8, 150mM NaCl, 1mM DTT. Actual concentration varies with lot number.
700 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-355
20S Proteasome
Product Source:
mouse
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes - a 20S core and a 19S regulator. This multi-subunit complex selectively degrades intracellular proteins. The 20S catalytic core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. This activity degrades a variety of peptide substrates and poly-ubiquitinated proteins with broad specificity in non-ATP dependent process. The 20S proteasome can be activated chemically by the addition of detergent or by the proteinaceous activator PA28 (E-380) or 19S (PA700)(E-366). The mouse proteasome enzyme is purified from mouse erythrocytes.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8, 150mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW:
700 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration for assay is 0.1-1 nM. 20S can be activated by SDS (0.035%) or with PA28 (Cat. # E-380).
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.