15-PGDH Inhibitor Screening Kit (Fluorometric)

Alternate Name : Prostaglandin-Endoperoxide Synthase 2 Inhibitor Screening Kit (Fluorometric), PTGS2 Inhibitor Screening Kit (Fluorometric)

Tag Line :

Simple assay to screen/study/characterize COX-2 Inhibitors

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em = 535/587 nm)
• Applications- Screening/studying/characterizing 15-PGDH inhibitors.

Detection Method: Fluorescence (Ex/Em = 535/587 nm)

Features & Benefits:

• Simple method to screen 15-PGDH inhibitors
• High-throughput Compatible
• Includes 15-PGDH Inhibitor Control,

Description:

15-hydroxyprostaglandin dehydrogenase (15-PGDH, EC: 1.1.1.141) catalyzes the oxidation of Prostaglandins (PG) to 15-keto metabolites which show greatly reduced biological activity when compared to Prostaglandins. 15-PGDH catalyzes the rate-limiting step of PG catabolism. More recently, the inhibition of 15-PGDH has been reported as a negative regulator of tissue repair and inhibition of this enzyme can potentiate tissue regeneration in mouse models. Thus, pharmacological inhibition of 15-PGDH could have clinical implications by accelerating potential tissue regeneration after damage. In this assay, 15-PG is oxidized by 15-PDGH generating 15-Keto metabolites and oxidizing NAD. NADH reduces a Fluorometric probe thus, generating an enhanced and stable fluorescence signal (Ex/Em=535/587 nm). In the presence of an inhibitor, the enzymatic activity is inhibited resulting No/Low fluorescence. BioVision’s 15-PGDH Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of 15-PGDH inhibitors.