10X Ubiquitin Conjugation Reaction Buffer
- 貨號 Cat. No.
- B-70
- 規格 Size
- 5 ml
- 品牌 Brand
- Boston Biochem
Description
10X Ubiquitin Conjugation Reaction Buffer
Product Information
5 ml
Use & Storage
Dilute to 1X in final reaction.
Store at -20°C. Avoid multiple freeze/thaw cycles.
Catalog #: B-70
10X Ubiquitin Conjugation Reaction Buffer
Ubiquitin reaction buffer contains the optimal formulation for the charging of ubiquitin(U-100) by E1 (E-305), the first reaction in the conjugation cascade. This E1-Ubiquitin thioester can be subsequently used for the initiation of any E1 mediated reaction including E2 ubiquitin thioester formation and/or the ubiquitination conjugation reaction of target substrate proteins with the addition of E2 / E3 enzymes. This buffer can supplement the fraction II HeLa conjugation (K-930) and fraction II reticulocyte conjugation (K-935), ubiquitin-protein conjugation (K-960), and ubiqiutin
thioester/conjugation (K-995) kits.
Product Information
Quantity:
5 ml
Supplied:
comes in 1ml x 5 vials (5ml total)
Use & Storage
Use:
Dilute to 1X in final reaction.
Storage:
Store at -20°C. Avoid multiple freeze/thaw cycles.